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human colon epithelial cell line  (ATCC)


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    ATCC human colon epithelial cell line
    Human Colon Epithelial Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 238 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human colon epithelial cell line/product/ATCC
    Average 97 stars, based on 238 article reviews
    human colon epithelial cell line - by Bioz Stars, 2026-03
    97/100 stars

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    SNHG26 is upregulated in colorectal cancer and knockdown inhibits proliferation. (A) Relative SNHG26 expression in colorectal cancer tissues compared to adjacent normal tissues across eight cohorts. (B) Relative SNHG26 expression in colorectal cancer cell lines (HT29, SW620, DLD1, HCT116 and SW480) and normal colon <t>epithelial</t> cell line <t>FHC.</t> (C) Knockdown efficiency of SNHG26 using two independent siRNAs (si‐SNHG26#1 and si‐SNHG26#2) in HCT116 and SW480 cells. (D, E) Cell proliferation assessed by CCK‐8 assay in HCT116 cells (D) and SW480 cells (E) after SNHG26 knockdown. (F, G) Colony formation assay results in HCT116 and SW480 cells following SNHG26 knockdown. Representative images (F) and quantification (G) are shown. (H, I) EdU incorporation assay showing DNA synthesis in HCT116 and SW480 cells after SNHG26 knockdown. Representative images (H) and quantification (I) are shown. (J‐K) Flow cytometry analysis of apoptosis in HCT116 and SW480 cells after SNHG26 knockdown. Representative plots (J) and quantification (K) are shown. * p < 0.05, ** p < 0.01, *** p < 0.001.
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    SNHG26 is upregulated in colorectal cancer and knockdown inhibits proliferation. (A) Relative SNHG26 expression in colorectal cancer tissues compared to adjacent normal tissues across eight cohorts. (B) Relative SNHG26 expression in colorectal cancer cell lines (HT29, SW620, DLD1, HCT116 and SW480) and normal colon epithelial cell line FHC. (C) Knockdown efficiency of SNHG26 using two independent siRNAs (si‐SNHG26#1 and si‐SNHG26#2) in HCT116 and SW480 cells. (D, E) Cell proliferation assessed by CCK‐8 assay in HCT116 cells (D) and SW480 cells (E) after SNHG26 knockdown. (F, G) Colony formation assay results in HCT116 and SW480 cells following SNHG26 knockdown. Representative images (F) and quantification (G) are shown. (H, I) EdU incorporation assay showing DNA synthesis in HCT116 and SW480 cells after SNHG26 knockdown. Representative images (H) and quantification (I) are shown. (J‐K) Flow cytometry analysis of apoptosis in HCT116 and SW480 cells after SNHG26 knockdown. Representative plots (J) and quantification (K) are shown. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: SNHG26 Promotes Colorectal Cancer Progression via CDKN2A ‐Dependent Regulation of Cuproptosis and CD8 + T Cell‐Mediated Immunity

    doi: 10.1111/jcmm.70913

    Figure Lengend Snippet: SNHG26 is upregulated in colorectal cancer and knockdown inhibits proliferation. (A) Relative SNHG26 expression in colorectal cancer tissues compared to adjacent normal tissues across eight cohorts. (B) Relative SNHG26 expression in colorectal cancer cell lines (HT29, SW620, DLD1, HCT116 and SW480) and normal colon epithelial cell line FHC. (C) Knockdown efficiency of SNHG26 using two independent siRNAs (si‐SNHG26#1 and si‐SNHG26#2) in HCT116 and SW480 cells. (D, E) Cell proliferation assessed by CCK‐8 assay in HCT116 cells (D) and SW480 cells (E) after SNHG26 knockdown. (F, G) Colony formation assay results in HCT116 and SW480 cells following SNHG26 knockdown. Representative images (F) and quantification (G) are shown. (H, I) EdU incorporation assay showing DNA synthesis in HCT116 and SW480 cells after SNHG26 knockdown. Representative images (H) and quantification (I) are shown. (J‐K) Flow cytometry analysis of apoptosis in HCT116 and SW480 cells after SNHG26 knockdown. Representative plots (J) and quantification (K) are shown. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Colorectal cancer cell lines (HCT116, SW480, SW620, HT29 and DLD1) and the normal colon epithelial cell line Fetal Human Colon (FHC) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Knockdown, Expressing, CCK-8 Assay, Colony Assay, DNA Synthesis, Flow Cytometry